Example of the method of touch up PCR

Touch up PCR is a method of amplifying a PCR-product that have bad primers or are troublesome in other ways. This is an example:

1. 98°C for 2:00 - Denaturing and warm up
2. 98°C for 0:10 - Denaturing after each cycle
3. 50°C for 0:30 - Annealing
   • Temperature increment of 1°C/cycle
4. 72°C for 2:00 - Elongation
5. Go to number 2 - repeat 10 times
6. Go to number 2 - repeat 5 times
7. 72°C for 5:00 - Final extension
8. End

For literature see([http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3313699/ link])