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From 2013.igem.org

NanoDrop data from yesterday's gel extraction

The NanoDrop machine was zero-ed using the elution buffer from the gel extraction kit used.

• Yellow pigment (BBa_K592010) - 6.6ng/ul
• Magenta pigment (BBa_K592012) - 4.9ng/ul
• Green light sensor, CcaS (BBa_K592001) - 3.2ng/ul
• RFP control - 4.4ng/ul
• New Part 2, promoter and RBS added to FixJ - 2.1ng/ul
• Blue light sensor, YF1 (BBa_K592004) - 1.4ng/ul
• FixJ protein intermediate, (BBa_K592005) - 10.0ng/ul
• Kanamycin resistant plasmid - 2.0ng/ul

Re-streak plating

We re-streak plated all of the plates that were more than 10 days old:

Magenta pigment (K592012)

OmpR promoter

RBS (B0034) PSB1A2

Terminator (B0015) PSB1L3

Blue sensor (K592004) PSB1C3

Promoter + RBS (K608002) PSB1C3

CcaS (K592001)

RFP control

FixJ intermediate (K592005)

CcaR

GFP

Yellow pigment (K592010)

• NOTE : The GFP and yellow pigment plates got muddled up before they were labelled and had to be streaked the next day (19th July).

• Note : When the RFP control had been incubated over night it had turned a magenta/red colour.

TO BE CONTINUED