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From 2013.igem.org

Goals & Challenges

Goals:

a.)our bacteria should express our proteins actively

b.)expressed proteins should isolated and purified effectively

c.)proteins should penetrate cell membrane

d.) induce apoptotic pathway on cancer cell spesifically.

Challenges:

a.) we needed end the life of cancer cells so we chosen apoptosis.Because cells may be eliminated by a number of alternative mechanisms including necrosis. Necrosis is typically described as a “nonspecific” form of cell death, characterized by rupture of the plasma membrane with a consequent localized inflammatory response and damage to surrounding cells and tissues. In contrast, apoptosis is associated with the rapid engulfment and removal of cell corpses by phagocytic cells that recognize “eat-me” signals displayed on the outer surface of the apoptotic cell . Furthermore, apoptotic cell death is the consequence of a series of precisely regulated events that are frequently altered in tumor cells. This provides the opportunity for selective clinical intervention to bring about the death of the tumor cell without damage to normal cells.These basic differences between these two processes of cell death underscore the reason why apoptosis, and not necrosis, represents the most desirable target mechanism for the induction of cell death in tumor cells.

b.)Chosen apoptotic proteins should act as a tumor specific. Despite the success of modern cancer therapies,the three major therapeutic interventions surgery,radiation, and chemotherapy still typically result in considerable damage to healthy tissue.We need new cancer treatments that precisely distinguish between diseased and healthy cells. To this end, synthetic biologists have engineered bacteria to target and invade cancer cells. Engineering bacteria in order to express tumor specific killer proteins enables . We aimed protective healthly cell untill thearapy. Thus we chosen apoptin.

c.) Also we cogitated that proteins alternative to apoptin so we found e4of4.It behave as similar apoptin.In this way we supported more data.

d.) Our constructs can not across membrane because of they are large molecules.So we used TAT peptide.It acts as a membrane shuttle; the structural determinants of transport and the manner by which the peptide crosses the lipid bilayer

e.)We offered that disjunctive penetrating peptide in stead of TAT.So we improve data. Also we selected MPG peptide.

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