Mirjam Made a PCR for silk 2 and 3 (strepF-R and F-strepR)in triplo - 28 ul MQ - 1.5 ul DMSO - 10 ul GC buffer - 1 ul of each dNTP - 2.5 ul primer F - 2.5 ul primer R - 0.5 ul phusion - 1 ul genomic DNA (BBa_...016)