Team:Groningen/Labwork/23 July 2013

From 2013.igem.org

Mirjam
Run a gel with the PCR products of silk without strep + signal sequence.
The PCR of silk without strep + signal sequence is successful. A new 1.5% agarose gel is made to do gel purification. Did the gel purification.

Run a gel with the purified PCR product of silk strepF-R and silk F-strepR.
The products are present in 2/3 samples for each silk type. These are combined and concentrated. Concentrations of 18.1 and 16.1 ng/ul are obtained. So a restriction digestion can be made.

Made a restriction digestion for the signal sequences.

Sander
Made a restriction digestion for silk strepF-R and silk F-strepR.