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From 2013.igem.org

Title

Project Title: Aromatics busted

Abstract

There exist various proteins which sense aromatic components and regulate the transcription of corresponding genes in prokaryotes, for example, Escherichia coli and Pseudomonas putida. However, naturally(natural) existing bio-sensors have various limitations including limited detection range, expression of leakage and low response ratio. This year our team designs a series of aromatic sensors and uses related enzymes in metabolic pathways to increase detectable aromatic compounds. We modify and characterize several existing biosensors. We also design a tunable band-pass gene circuit, which enables E.coli containing certain sensor respond to a specific inductor concentration. Combining the method of gradient dilution, we can measure the concentration of aromatic components in a convenient way. In the long run, these bio-sensors and promoters enrich the inducible device library in synthetic biology.

Project Abstract and Title

Project Title: Aromatics Busted

Project Abstract: There exist various proteins which sense aromatic components and regulate the transcription of corresponding genes in prokaryotes, for example, Escherichia coli and Pseudomonas putida. However, naturally(natural) existing bio-sensors have various limitations including limited detection range, expression of leakage and low response ratio. This year our team designs a series of aromatic sensors and uses related enzymes in metabolic pathways to increase detectable aromatic compounds. We modify and characterize several existing biosensors. We also design a tunable band-pass gene circuit, which enables E.coli containing certain sensor respond to a specific inductor concentration. Combining the method of gradient dilution, we can measure the concentration of aromatic components in a convenient way. In the long run, these bio-sensors and promoters enrich the inducible device library in synthetic biology.