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From 2013.igem.org

Results

Verification gel

1% gel to verify our earlier PCR purifications

wells (in bracets position of the purification in the gene box):

• 1: broad band ladder
• 2: HAO extracted from N. europeae with flanking primers (A4)
• 3: AMO extracted from N. europeae with flanking primers (A6)
• 4: cycAX extracted from N. europeae with flanking primers (A5)
• 5: Nir extracted from P. aeruginosa with flanking primers (A7)
• 6: Nir extracted from P. aeruginosa with flanking primers (A8)
• 7: Nir extracted from P. aeruginosa with flanking primers (B8)
• 8: Nir extracted from P. aeruginosa with flanking primers (B9)
• 9: pZA21 USER fragment, DpnI treated (F1)
• 10: cycAX USER fragment (G1)
• 11: AMO USER fragment (G2)
• 12: HAO USER fragment (G3)
• 13: Nir USER fragment (G4)
• 14: AMO USER fragment (G5)
• 15: 1kb plus ladder

colony PCR gel