Team:DTU-Denmark/Notebook/24 July 2013
From 2013.igem.org
Contents |
208
Main purpose
Who was in the lab
Henrike, Julia, Gosia, Krystian
Procedure
USER reaction and transformation
We perform USER reaction in order to create construct of plasmid pZA21 and AMO and other construct - pZA21 and HAO. We add also negative control with water instead of insert. Reaction is performed according to our protocol with increased amount of insert.
Restriction analysis
Primer diluting
PCR to extract Nir from Pseudosomonas with new USER primers
Results
Conclusion
Navigate to the Previous or the Next Entry