Team:DTU-Denmark/Notebook/23 July 2013
From 2013.igem.org
22 July 2013
Contents |
208
Main purpose
- ON culture of Colony PCR products of HAO and AMO from 19-07-2013
- USER reaction of AMO and HAO with pZA21 (with native promoter).
- RFP amplification in pZA21 using primers without promoter
- PCR to amplify USER-compatible AMO and HAO fragments
Who was in the lab
Gosia, Kristian, Henrike
Results
Verification gel
1% gel to verify our earlier PCR purifications
wells (in bracets position of the purification in the gene box):
- 1: broad band ladder
- 2: HAO extracted from N. europeae with flanking primers (A4)
- 3: AMO extracted from N. europeae with flanking primers (A6)
- 4: cycAX extracted from N. europeae with flanking primers (A5)
- 5: Nir extracted from P. aeruginosa with flanking primers (A7)
- 6: Nir extracted from P. aeruginosa with flanking primers (A8)
- 7: Nir extracted from P. aeruginosa with flanking primers (B8)
- 8: Nir extracted from P. aeruginosa with flanking primers (B9)
- 9: pZA21 USER fragment, DpnI treated (F1)
- 10: cycAX USER fragment (G1)
- 11: AMO USER fragment (G2)
- 12: HAO USER fragment (G3)
- 13: Nir USER fragment (G4)
- 14: AMO USER fragment (G5)
- 15: 1kb plus ladder
colony PCR gel
1% gel to analyse yesterday's colony PCR
wells:
- 1: 1 kb ladder
- 2: HAO colony 1
- 3: HAO colony 2
- 4: RFP in pZA21 without promoter
- 5: RFP in pZA21 without promoter
- 6: AMO colony 3 (count starts from 3)
- 7: AMO colony 4
- 8: AMO colony 5
- 9: AMO colony 6
- 10: AMO colony 7
- 11: AMO colony 8
- 12: AMO colony 9
- 13: AMO colony 10
- break in the gel
- 14: cycAX colony 1
- 15: cycAX colony 2
- 14: RFP colony 1
- 14: RFP colony 2