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Contents 1 Background 1.1 Overview 1.2 Growing Desert 1.3 Threatened Desert 2 Wet Lab 2.1 Overview 2.2 The Parts 2.3 Data and Results

Background

Overview

Growing Desert

Threatened Desert

Wet Lab

Overview

In the past other teams (Osaka, University College London) have explored the resistance genes in the bacteria D. radiodurans. These resistance genes convey resilience against high levels of radiation, oxidative stress and desiccation in that the DNA repairs itself when damaged from these stressors. The interesting thing about these stressors is that the DNA repairs itself in the same way regardless of the type of stress. Simply put, the system responds to DNA damage and makes the necessary repairs. From here, the project first aims to provide extra validation data to those studies. Additionally the ultimate purpose of this iGEM project is to explore the resistance genes in a similar bacteria, Deinococcus hopiensis, as a novel source of these genes within the Deinococcus genus. For this purpose the project then uses the genes to transform a desiccation, radiation, and oxidative stress sensitive bacteria, E. coli to improve the robustness of the cells against the particular stressors of desiccation and oxidative stress. In science, adding validation data is always a very important and welcome resource. In addition to the validation data, the main goal of the project is to look for these same genes in a sister bacteria and see if those genes convey the same resistance.

The Parts

Data and Results