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From 2013.igem.org

Title

Project Title: A b

Abstract

There exist various proteins which sense aromatic components and regulate the transcription of corresponding genes in prokaryotes, for example, Escherichia coli and Pseudomonas putida. However, naturally(natural) existing bio-sensors have various limitations including limited detection range, expression of leakage and low response ratio. This year our team designs a series of aromatic sensors and uses related enzymes in metabolic pathways to increase detectable aromatic compounds. We modify and characterize several existing biosensors. We also design a tunable band-pass gene circuit, which enables E.coli containing certain sensor respond to a specific inductor concentration. Combining the method of gradient dilution, we can measure the concentration of aromatic components in a convenient way. In the long run, these bio-sensors and promoters enrich the inducible device library in synthetic biology.

Project Abstract and Title

Project Title: Aromatics Busted

Project Abstract: There exist various proteins which sense aromatic components and regulate the transcription of corresponding genes in prokaryotes, for example, Escherichia coli and Pseudomonas putida. However, naturally(natural) existing bio-sensors have various limitations including limited detection range, expression of leakage and low response ratio. This year our team designs a series of aromatic sensors and uses related enzymes in metabolic pathways to increase detectable aromatic compounds. We modify and characterize several existing biosensors. We also design a tunable band-pass gene circuit, which enables E.coli containing certain sensor respond to a specific inductor concentration. Combining the method of gradient dilution, we can measure the concentration of aromatic components in a convenient way. In the long run, these bio-sensors and promoters enrich the inducible device library in synthetic biology.