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Team:Groningen/Labwork/26 July 2013
From 2013.igem.org
Mirjam
Made a gel to check the gel purified products of Pdes, CheY and des DOWN. These products look fine. A check of the PCR products of CheY UP, spec and CheY DOWN revealed that the PCR for spec failed again.
Gel purification is done for CheY UP, CheY DOWN and Tet.
New PCR reactions are made for all types of silk we want to create. This time the bp time is increased to 30 sec, to examine if this helps to obtain a higher concentration.
Did a restriction digestion with BamHI for Pdes and CheY.
Claudio
The Bacillus Subtilis plates incubated overnight show colonies.
One colony is picked and transformed with the new plasmid derived from iGEM Munich 2012.
The transformants are plated on:
- LB agar + chloramphenicol
- LB agar + chloramphenicol + IPTG
iGEM 2013 Groningen
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