Team:Groningen/protocols/PCR

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PCR

Materials:

PCR tubes
F- and R-primer
DNTPs
Phusion buffer
H₂O
Phusion Polymerase
DNA template

Steps:

Component	50μl reaction	Final concentration
H₂O	up to 50μl
5x Phusion Buffer	10	1x
10mM dNTPs	1	200μM
F-primer	1	0.2μM
R-primer	1	0.2μM
Template DNA	1μl	~1ng
Phusion Pol.	0.5μl	0.02U/μl

Cycling instructions:
Reference: http://www.thermoscientificbio.com/uploadedFiles/Resources/f-530s-product-information.pdf