Gel electrophoresis

Materials:

• Power supply
• Agarose gel
• Tray
• DNA samples
• DNA Ladder

Procedure:

All the DNA samples are mixed 1:1 ratio with Loading Dye 6x.
The samples are loaded on agarose gel 0.8% or 1.5% (diluted in TBE buffer 1x).
Beside the samples the DNA ladder is loaded.
90V electric potential is applied to the gel as long as the bands are apart from each other (30-45 minutes).
The gel are run in TBE buffer 1x.