Gel electrophoresis

Materials:

• TBE buffer 1x
• 0.8 or 1.5% agarose gel
• 2x Loading Dye
• DNA samples
• DNA 1kb GeneRuler of Fermentas

Procedure:

- Mix the DNA samples in a 1:1 ratio with 2x Loading Dye
- Load the samples on a 0.8% or 1.5% agarose gel
- Load the 1kb GeneRuler of Fermentas on gel
- Run the gel at 90V for 24-45 minutes to separate the bands.