Team:Groningen/Labwork/1 August 2013

Mirjam

Plates for the transformation of ΔDes, ΔCheY and ΔDes/ΔCheY did not show any colonies. The plates for eYFP showed some colonies on the plates with the transformation to E.coli. The plates with RFP and the ligation of Pdes/Chey did not show any colonies. Also the plates with a transformation of GFP240 and GFP0840 showed colonies.
Inoculated the colonies into LB with ampicillin to perform a miniprep later on.
Made a new restriction digestion for RFP, eYFP and BBa_k823823 + LacI with EcoRI and PstI. Did a gel purification to obtain the purified fragment.
Inoculated B.subtilis colonies for transformation.
Made a restriction for Pdes with EcoRI and BamHI, for CheY with BamHI and PstI and for BBa_k823823 with EcoRI and PstI.

Chaline

Sebas

Sander

Made a plasmid purification of GFP BBa_E0240 and got a concentration of 21,5 and also did a purification of GFP BBa_E0840 and got a concentration of 13,5.(placed in temp box labeled as GFP 0240 and GFP 0840)