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Team:Groningen/Labwork/2 August 2013
From 2013.igem.org
Claudio
The plates prepared yesterday show colonies.The strains inoculated by Inne grew. -80°C stock of AKP4 (Δdes::kn::cm) and OIB055 (ΔcheY::cm) was made and stored in the box labeled iGEM 2013.
BBa_K823023 + lacI, BBa_E0840 and BBa_E0240 are digested with EcoRI and PstI. The samples are purified using the PCR purification kit. BBa_K823023 + lacI is ligated to BBa_E0840 and BBa_E0240 (3:1 insert:vector ratio). The ligation products are transformed to E. Coli DH5α and the competent cells are plated on LB + Amp.
iGEM 2013 Groningen
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