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Team:Groningen/Labwork/3 August 2013
From 2013.igem.org
Claudio
On the 2x SG plate, which was dried over night at 37°C, 2µl of overnight Bacillus Subtilis culture is spotted.The plates (plated yesterday) show colonies. A few colonies are picked and streaked out on LB + Amp + IPTG (100µM).
The plasmid (isolated by Sander) that seems to contain the desired construct is transformed in E. Coli DH5α and plated on LB + Amp + IPTG (100µM).
iGEM 2013 Groningen
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