lab 208

Main purpose

Who was in the lab

Kristian, Gosia, Natalia, Henrike

Procedure

PCR

lots of PCR done

gel purification

of the Nir1 fragment extraced from P. aeruginosa

plasmid miniprep

HAO in pZA21 from USER cloning was purified for restriction analysis

Results

1% agarose gel

• 1 kb ladder
• Nir1 col. T
• Nir1 col. T
• Nir2 col. T
• Nir2 col. T
• Nir1 frag. T
• Nir1 frag. T
• Nir1 col. X7
• Nir1 col. X7
• neg
• Hi
• Hi
• DMSO
• DMSO
• Nir1
• Nir1
• Nir2
• Nir2
• 1 kb ladder

1% agarose gel

• 1 kb ladder
• NirW
• NirW
• araBAD biobrick K808000
• araBAD biobrick K808000
• SPL in pZA21 containing RFP
• SPL in pZA21 containing RFP
• reference promoter in pZA21 containing RFP
• reference promoter in pZA21 containing RFP
• neg
• neg
• 1 kb ladder

(pic small gel)

Conclusion

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