Team:Groningen/Labwork/7 August 2013

Mirjam

A check on the plates with CFP revealed that no colonies are present.
A check on the plates for ΔCheY revealed colonies. The ΔDes plates did not reveal any colonies.
Check of the restriction digest made 06-08 of CheY, and the two tubes of CFP. This reveals that CheY and one of the CFP tubes is totally absent.
Made a restriction digestion of GFP/YFP with Xba and PstI and did gel purification.
Made a restriction digestion of CFP with EcoRI and PstI and did gel purification.
Did a gel purification on BBa_k823823 restricted with EcoRI and SpeI.
Made a restriction digestion of CheY with BamHI and SpeI. Heat inactivated the reaction.

Chaline

Measured the concentration of three mini prep tubes of CFP and one of the restriction digests.
Measured the concentration of the restricted GFP, YFP and BBa_k823823.
Run a gel with the (purified) restrictions of BBa_k823823, CFP, GFP, YFP and CheY.

Claudio

The Bacillus Subtilis biofilm grew on 2x SG medium after 4 and 5 days of incubation respectively. Liquid 2x SG broth is prepared in order to grow biofilm between liquid-air interface.