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Team:Groningen/Labwork/8 August 2013
From 2013.igem.org
Mirjam
Check of the plates of the restrictions made yesterday. One of the plates with CFP showed 1 colony. The other two plates (one IPTG) did not show any. The transformation of Pdes/CheY into the backbone failed, but the transformation of Pdes/CheY/eYFP showed a couple of colonies.Inoculated the colony of CFP and four colonies of Pdes/CheY/eYFP in LB medium with amp for mini prep.
Made a new ligation reaction between Pdes and CheY.
Made a new ligation reaction between CFP and the backbone.
Made a new ligation reaction between Pdes/CheY and the backbone.
Transformation to E. coli of all the ligation reactions.
Claudio
The biofilm grew during the last few days
iGEM 2013 Groningen
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