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Risk Group and Biosafety Level Containment
This table contains NIH guidelines to organism Risk Group and Biosafety Containment Level. It should help you determine what BSL level you need to be working in and what RG your organisms are.
| Risk Group |
Biosafety Containment Level |
Examples |
| Risk Group 1: Agents are Not associated with disease in healthy adult humans. (Low risk) |
BSL-1
- Work is done on open bench tops and special containment
equipment is not required.
- Standard microbiological practices are observed.
- Biohazard signs should be posted.
|
Escherichia coli; K12 derivatives (DH5a, JH109, pBluescript, psi2) |
| Risk Group 2: Agents are associated with human disease which is rarely serious. There are often preventive or therapeutic interventions available. (Moderate risk) |
BSL-2
- All BSL-1 containment and practices plus the following:
- Laboratory access is restricted when experimental work is in progress
- Personnel have specific training in handling of agents
- Biological safety cabinets (BSC) or other physical containment devices are used for potential aerosol generation procedures
- Biohazard signs must be posted
- Specific PPE (personnel protective equipment) and entrance requirements
|
- Adenovirus all types; human
- All human blood contaminated specimens; HIV/SIV infected animals
- Human cell lines eg. HEK 293, HeLa
- Herpes Simplex Virus
- Rabies Virus
- Pseudorabies Virus
|
| Risk Group 2 or Risk Group agents; Protocol specific (Moderate to High risk) |
BSL-2+ ("Plus")
- All BSL-2 facilities, containment and practices PLUS BSL-3 practices including:
- Restricted Laboratory access
- Personnel have specific training in handling of agents
- All procedures are performed in biological safety cabinets (BSC)
- Biohazard signs must be posted
- Written safety policies provided by the investigator defining laboratory procedures, waste disposal, disinfection and medical surveillance
- Centrifuge safety cups must be used
|
- Lentiviral vectors, such as:
- SIV; proviral DNA
- HIV-1 infectious molecular clones
|
| Risk Group 3: Agents are associated with serious or lethal human disease for which preventive or therapeutic interventions MAY be available. (High risk) |
BSL-3
- NO current facilities exist to accommodate risk group 3 agents at iGEM
|
None; no current usage of this risk group classification at iGEM |
| Risk Group 4:Agents are likely to cause serious or lethal human disease for which preventive or therapeutic interventions are NOT USUALLY available. (Extreme risk) |
BSL-4
- NO current facilities exist to accommodate risk group 4 agents at iGEM
|
None: no current usage of this risk group classification at iGEM |
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