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Team:ITU MOBGAM Turkey/project
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As an iGEM team, our aim is to synthesize and secrete intrinsic factor (IF) protein in ileum by using E. coli. We determined our IF production and secretion location as ileum instead of stomach due to possible negative effects of low pH value in stomach. Because ileum is already serves as the location for binding of IF with B12 vitamin and absorption, choosing ileum will be advantageous. In addition to this, the possibility of occurrence of an autoimmune disease will be eliminated by providing E. coli its natural flora, which is ileum.
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