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From 2013.igem.org

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• iGEM 2013

Contents 1 March 2 April 3 May 3.1 5/1/13 3.2 5/2/13 3.3 5/3/13 3.4 5/4/13 3.5 5/5/13 3.6 5/6/13 3.7 5/20/13 4 June 5 July 6 August 7 September

March

April

May

5/1/13

LP, XL

- Commencement of spring!!!

- Discussed goals and outlined plans for spring term

5/2/13

LP, XL

- Designed primers for amplifying and sequencing phage capsid protein

- Wrote emails inquiring mutagen (NG) and plasmid for in vitro assembly

5/3/13

LP, XL

- Performed agar test, focusing primarily on ×8

5.3 T7 phage selection method test

- Processed phage amplification

5.3 T7 phage amplification/purification

5/4/13

LP, XL

- Performed dilution series using stock 5.3 (-1 through -11)

- Started two 5mL overnights of BL21

5/5/13

LP, XL

- Spot test using stock 5.3 and its dilution series (from 5.4)

5.3 T7 phage amplification/purification

- Started liquid culture for purification team (at around noon)

1mL of BL21 overnight + 4mL of LB + 100μL -2 phage (received stock)

- Started 5.5 amplification from a plaque test

5.5 Amplification from a plaque test

5/6/13

- Continued amplification from a plaque test

5.5 Amplification from a plaque test

- Performed liquid culture phage concentration test

5.6 T7+ Liquid Culture Phage Concentration Test

5/20/13

LP, XL

- We performed T7 Mutagen Concentration Test

5.20 Mutagen Concentration Experiment

- We performed T7 Minor Capsid Protein PCR

5.20 T7 Minor Capsid Protein PCR

June

July

August

September