Team:Glendale CC AZ/Protocols/SurivalGrowth

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Survival Growth Assay

Materials

   -P200 micropipette
   -10 microliters bacteria
   -90 mictroliters LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37 degrees celcius.

Procedure

1. Label all plates (Keep upside down to avoid condensation on agar) 2. Pipette LB media into tube containing bacteria; aspirate. 3. Using P200 micropipette, extract all 100 microliters of solution and place onto appropriate agar plate. 4. Briefly flame the spreader and let cool momentarily. 5. Spread bacteria-LB solution all over plate. 6. Incubate at 37 degrees celcius for up to 24 hours.