Team:Glendale CC AZ/Protocols/Ligation

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Ligation Protocol

Protocol from: http://parts.igem.org/Help:Protocols/Ligation

Materials 

  -digested plasmid backbone
  -equimolar amount of EcoRI-HF SpeI digested fragment
  -equimolar amount of XbaI PstI digested fragment
  -T4 DNA ligase buffer
  -T4 DNA ligase
  -dH2O

Procedure

Retrieved from "http://2013.igem.org/Team:Glendale_CC_AZ/Protocols/Ligation"