Team:Glendale CC AZ/Protocols/Transformation

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Transformation Protocol

Protocol from: http://parts.igem.org/Help:Protocols/Transformation

Materials

  -Resuspended DNA
  -Competent cells (50ul per transformation)
  -Ice
  -2ml tube (1 per a transformation)
  -42ºC water bath
  -SOC media
  -Petri dishes with LB agar and appropriate antibiotic (2 per transformation)
  -Spreader
  -37ºC incubator
  -10pg/ul RFP Control (pSB1A3 w/ BBa_J04450)

Procedure

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