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From 2013.igem.org

Survival Growth Assay

Materials

   -P200 micropipette
   -10µl bacteria
   -90µl LB media
   -Agar plates with appropriate antibiotic
   -Spreader
   -20mL ethanol (to sterilize spreader)
   -Incubator set to 37 degrees celcius.

Procedure

1. Label all plates (Keep upside down to avoid condensation on agar)

2. Pipette LB media into tube containing bacteria; aspirate.

3. Using P200 micropipette, extract all 100µl of solution and place onto appropriate agar plate.

4. Briefly flame the spreader and let cool momentarily.

5. Spread bacteria-LB solution all over plate.

6. Incubate at 37 degrees celcius for up to 24 hours.