Team:BostonU/Protocols

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Protocols

<h7>This section contains the protocols that we used during the summer and that are mentioned in our Notebook. We hope they help anyone interested in our experiments to successfully repeat them.

<a href="https://static.igem.org/mediawiki/2012/0/0b/Phusion_PCRblank.xls">Phusion PCR </a>

<a href="https://static.igem.org/mediawiki/2012/c/cb/Transformation_Gold_Bioline_cells.xls">Transformation with Gold Bioline Cells</a>

<a href="https://static.igem.org/mediawiki/2012/0/05/Restriction_Digest.xls">Restriction Digest </a>

<a href="https://static.igem.org/mediawiki/2012/8/8e/Qiagen_QIAquick_Gel_extraction.xls">Gel Extraction </a>

<a href="https://static.igem.org/mediawiki/2012/5/58/Qiagen_QIAprep_Miniprep.xls">Miniprep </a>

<a href="https://static.igem.org/mediawiki/2012/7/7e/NanoDrop_DNA_Quantification.xls">DNA Quantification </a>

<a href="https://static.igem.org/mediawiki/2012/b/bd/Media_Preparation_LB_broth_agar_Gels.xls">Media Prep </a>

<a href="https://static.igem.org/mediawiki/2012/1/1f/Ligation_Regular_and_MoClo.xls">Ligation </a> </html>