lab 208

Main purpose

1. PCR reaction in order to amplify backbone pZA21 containing araBAD and RFP
2. PCR reaction in order to amplify Nir1 and Nir2 fragments

Who was in the lab

Henrike, Kristian, Gosia

Procedure

1. PCR mix for backbone was made according to standard protocol.

• Primers - 1an and 1b
• Template - "Ara" sample (plasmid isolation pZA21:araBAD:RFP).
• Program was based on standard program with 65 C of annealing temperature and 4 min of extension time.
• Samples names: 1, 2, 3 and N (negative)

1. PCR for Nir1 and Nir2

Results

Conclusion

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