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From 2013.igem.org

Contents 1 Safety Database 2 EtBr 3 Solid Disposal 4 Liquid Disposal 5 General decontamination 6 Electrophoresis machine 7 Proper Bunsen burner technique 8 Appropriate PPE

Safety Database

EtBr

[www.purdue.edu/rem/hmm/ethidbr.htm]

• Ethidium bromide(EtBR) is a known toxin and mutagen. Its use should be limited and it should be handled with EXTREME CARE.
• Only use EtBr in designated areas clearly marked “EtBr contamination” to avoid contaminating other lab areas and equipment.
• Any PPE that has been contaminated with EtBr must be disposed of in an EtBr container
  • Latex gloves should not be worn when working with EtBr. Nitrile gloves are sufficient when minimal exposure time is expected. For longer periods of time, use multiple layers or chemically-resistant gloves.
  • Wash hands thoroughly and immediately after disposing of gloves
• If accidental exposure occurs:
  • Eyes- rinse with water for 15 minutes
  • Skin- wash the contaminated area with soap and water
  • Clothing- remove and place in sealed bag
  • ALWAYS seek medical attention immediately after an accident has occurred
• Methyl Blue alternative- not nearly as hazardous, but may not be as effective as EtBr

Solid Disposal

Liquid Disposal

General decontamination

Electrophoresis machine

Proper Bunsen burner technique

Appropriate PPE