lab 208

Main purpose

• PCR for extraction of Nir2 from PAO1

Who was in the lab

Gosia, Julia, Henrike

Procedure

PCR

PCR reaction for amplification of Nir2 extraction fragment in order to obtain a huge amount to use as template to make a screening PCR for the Nir2 USER fragment

primers: template: P. aeruginosa cells resuspended in 100 uL program: touchdown PCR (called U on Eppendorf)

Gel purification

gel purify PCR products from constitutive promoter SPL and constitutive promoter ref

Results

Conclusion

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