Team:Groningen/Labwork/14 August 2013

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Mirjam

Ligation reaction of ΔCheY and ΔDes (with help of Chaline) Transformation to B. subtilis ΔDes transformed in ΔCheY and ΔCheY into ΔDes. Inoculation of Pdes-CheY-GFP in LB medium with ampicillin (although strange colonies grew on the negative control plate). The colonies didn't grew in liquid LB medium.

Sander

made 3 inoculations of the cheY knockout and 3 inoculations of wild type bacillus in 0,4% agar with LB medium to test motility.

Chaline

Did a restriction on CheY down and Des up with EcoRI and heat inactivated the samples.
Run the samples over gel and the bands are visible.
Measured the concentration of CheY down and Des up.