"
Exeter/8 August 2013
From 2013.igem.org
Revision as of 09:46, 15 August 2013 by FelicityHeath (Talk | contribs)
Liquid Cultures, Miniprepped
- K592012 (Magenta) CAM
- RFP control CAM
- R0082 (OmpF promoter) CAM
- DH5α 3 (Tom and Adam's control) TET
- DH5α 5 (Tom and Adam's RBS + Cyan) TET
- DH5α 1 (Tom and Adam's RBS + cph8) TET
- S05058 (RBS + yellow) CAM
- K592010 (yellow) CAM
- K592018 (RBS + cph8) CAM
- K608002 (RBS + promoter) CAM
- B0015 (Terminator) CAM
- K322124 (RBS + cph8) CAM
( 3 replicates, #2, #3 and #4.)
NanoDropping Promoter + RBS Replicates
1 = 29.6ng/ul
1 = 29.2ng/ul
2 = 12.2ng/ul
2 = 12.5ng/ul
3 = 22.8ng/ul
3 = 23.6ng/ul
4 = 17.8ng/ul
4 = 17.5ng/ul
NanoDrop of liquid cultures
| Liquid culture | NanoDrop result (ng/ul) |
|---|---|
| Magenta | 106.9 |
| RFP | 42.9 |
| OmpF | 117.3 |
| DH5α 3 | 46.3 |
| S05058 | 67.1 |
| Yellow | 57.1 |
| RBS + cph8 | 113.7 |
| cph8 #4 | 49.2 and 48.8 |
| cph8 #2 | 221.9 and 180.7 |
| cph8 #3 | 70.8 and 70.8 |
| Promoter and RBS | 99.9 |
| B0015 | 52.1 |
| AMP plasmid | 82.5 |
We sent off cph8 K322124 red light sensor for sequencing.
2 replicates each of #4, #3 and #2 from glycerol stocks were done. 10ul in Eppendorfs.
