15/08/13

Revision as of 13:29, 15 August 2013 by AliceHaworth (Talk | contribs)

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Serial dilution of herring sperm DNA

100ul DNA and 900ul TE to make master mix
5 independent samples 10ul each were taken from master mix
- Each independent sample was diluted twice in 90ul of TE
20 dilution samples were then nanodropped to check dilution accuracy

Sample nr	Concentration (ng/ul)	260/280	260/230
nr. 1/1	529.9	1.81	2.26
nr. 1/2	52.1	1.86	2.32
nr. 2/1	454.3	1.81	2.28
nr. 2/2	46	1.82	2.23
nr. 3/1	457.3	1.83	2.28
nr. 3/2	45.1	1.85	2.38
nr. 4/1	522.8	1.82	2.27
nr. 4/2	47.3	1.87	2.26
nr. 5/1	481.4	1.82	2.28
nr. 5/2	40.8	1.84	2.35 Dilutions show accuracy Master dilution is sonicated and every 30mins a 30ul sample is taken to dilute twice (10ul of DNA in 90ul of TE buffer) 6 30ul samples are taken Samples are then run on a gel