Team:DTU-Denmark/Methods/Ammonium colorimetric measurements
From 2013.igem.org
Ammonium colorimetric measurements
Ammonium colorimetric measurements
Measuring the concentration of NH4 in an E. coli/P.aeroginosa growth experiment using Spectroquant kit
Material:
- Ammonium Test Kit (#00683)
A liquid test solution should be performed to achieve a measurement between the ranges of 2-75 mg/L NH4-N.
Ammonium solution
Prepare solution with 39 mg NH4-N/L:
- Weigh 220 mg Ammonium Bicarbonate into 100mL volumetric flask (10x concentrated)
- Take 5 mL of the 10x solution in 45 mL water
- Expected final concentration 39 mg N/L
Performing Measurements: Ammonium
Measuring range of the kit: 2-75 mg/L NH4-N
- Pipette 5.0 ml of reagent 1 (NH4-1) into a test tube.
- Add 0.20 mL of sample when it is among the range of measuring kit (otherwise make proper dilution)
- Add 1 level spoon of reagent 2 (NH4-2).
- Shake vigorously to dissolve.
- Reaction time: 15 mins
- Pour sample into the cuvette
- Select method in the spectrometer by entering the AutoSelector (little black tube)
- Note the result
- If the read is above the linear range then put the sample back to the tube test, dilute it and try again.