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Team:DTU-Denmark/Notebook/16 August 2013
From 2013.igem.org
16 August 2013
Contents |
lab 208
Main purpose
Who was in the lab
Kristian, Julia, Henrike
Procedure
Gradient PCR for Nir2 USER
Set up new gradient PCR for Nir2 USER to run in 223. Reaction mix without MgCl2 but using DMSO and GC-buffer.
template: Nir2 extraction fragment
primers: 40a, 40b
additives: 5% DMSO
buffer: GC
Gradient went from 60C to 72C in 12 steps.
| sample # | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| temperature | 60.2 | 60.5 | 61.6 | 63.0 | 64.2 | 65.4 | 66.6 | 67.6 | 68.9 | 70.2 | 71.6 | 72 |
Results
Conclusion
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