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- Overview
- Winter
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- Fall
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1. Goals for the week
2.Experiments and results
5.9 T7+ Liquid Culture Phage Concentration Test #2
- We infected E. coli BL21 with different concentrations (1ul, 10ul, and 100ul) of phage to see which would yield the highest titer. Plaques formed up to -8 on all the spot tests, showing that the concentration difference had no effect on the titer. However, the plates were badly contaminated.
5.13 Determining E. coli Concentration With Spectrophotometer
- To check if we could determine E. coli concentration with a spectrophotometer, we created a 7/5 dilution series of E. coli. We then measured the absorption at 600nm of each E. coli dilution. When plotted, these measurements showed a linear relationship between E. coli concentration and the absorption reading.
3. Next Steps
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