"

From 2013.igem.org

{ "date" : "2013-08-13", "author" : "viola-thomas", "title" : "Triple short ligation of Plambda promoter, EFE+terminators in pSB1C3", "content" : "Today we added SAP to psb1c3 and DpnI to S04617 and to EFE+B0015. We followed the short cloning protocol so we didn't purified the digestion products and, after the deactivation of the enzymes used , we proceeded with the ligation following the standard quantities. We stimate the same lenght for both the inserts (EFE and s04617).

", "tags" : "pFixK-cI-pLambda-EFE" }