"

From 2013.igem.org

{ "date" : "2013-08-15", "author" : "emil", "title" : "Emil's(re)begins", "content" : "I screened 5µl of the two PCR that I started the day before(5 of K823025 and 2 of E0840, These are the results:

Unfortunately there isn't the ladder I loaded only the pure dye, otherwise can reasonably consider the PCR correct, after this I purified 3 backbone and the 2 GFP following the purification protocol with the following results:

     
results

         
            GFP 170.5 ng/6micro;lGFP 252 ng/6micro;lPXyl 1110.4 ng/6micro;lPxyl 277.7 ng/6micro;lPxyl 3137 ng/6micro;l

                

                   
Sample
                   
Quantities
                
                

                    

                    

                
                

                    

                    

                
                 

                    

                    

                
                 

                    

                    

                
                 

                    

                    

                
             
           
        
   After that I did a digestion overnight of 3 µl of the GFP1 and the PXyl1 following the  digestion protocol.",

"tags" : "PXyl-GFP" }