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Team:Groningen/Labwork/15 August 2013
From 2013.igem.org
Inne
Plates that were made yesterday showed no difference betweeen the supposed motile and no motile bacteria.New plates are made with agar concentration of 0.4%, 0.2% and 0.1% to see if the concentration of agar could be the problem.
Plates are to dry overnight.
Sebas
Did a colony PCR op deltaDES/CheY tet with primers HM07&HM10 (Fw tet with Rev downstream des) colony 2 had the right insert. Inoculated 3ml LB with colonie 2. Did a miniprep on the other backbone with GFP and digested it with XbaI and PstI. Digested the hyperspank backbone with X and P and did a ligation O/N in a beaker with RT water in the fridge.
iGEM 2013 Groningen
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