From 2013.igem.org
""m&m""
Aug 20
Miniprep
| DNA | concentration[μg/μL] | 260/280 | 260/230
|
| 8/18 Ptet -1 | 220 | 1.69 | 1.87
|
| 8/18 Ptet -2 | 210 | 1.69 | 1.71
|
| 8/18 RBS-tetR-DT -1 | 236 | 1.63 | 1.69
|
| 8/18 RBS-tetR-DT -2 | 182 | 1.65 | 1.98
|
| 8/18 J23100-RBS-GFP-DT -1 | 334 | 1.71 | 2.12
|
| 8/18 J23100-RBS-luxR-DT -1 | 440 | 1.67 | 1.60
|
| 8/18 J23100-RBS-luxR-DT -2 | 344 | 1.68 | 1.83
|
| 8/18 J23100-RBS-lacZα-DT -1 | 280 | 1.70 | 1.81
|
| 8/18 RBS-lysis3 -1 | 282 | 1.67 | 1.76
|
| 8/18 RBS-lysis3 -2 | 212 | 1.30 | 1.35
|
LB Culture Medium
volume - 200mL
| name | amount
|
| Bacto Trypton | 2g
|
| Bacto yeast extract | 1g
|
| Nacl | 1g
|
| Agar Powder | 2g
|
| Amp | 40μl
|
1.Measuring reagents and put they in the Erlenmeyer flask
2.diluting in measuring cylinder to 200ml total
3.autoclaving
4.adding ampicirine 40ml
not written yet
Ristriction Enzyme Proccessing
| 8/20 Ptet -1(220μg/mL) | EcoRI | XbaI | 10x BSA | 10x buffer M | MilliQ | total
|
| 4.5 | 1 | 1 | 3 | 3 | 17.5 | 30
|
| 0.5 | 0.2 | 0 | 1 | 1 | 7.3 | 10
|
| 0.5 | 0 | 0.2 | 1 | 1 | 7.3 | 10
|
| 0.5 | 0 | 0 | 1 | 1 | 7.5 | 10
|
| 8/20 RBS-tetR-DT -2(182μg/mL) | EcoRI | SpeI | 10x buffer H | MilliQ | total
|
| 5.5 | 1 | 1 | 3 | 19.5 | 30
|
| 0.6 | 0.2 | 0 | 1 | 8.2 | 10
|
| 0.6 | 0 | 0.2 | 1 | 8.2 | 10
|
| 0.6 | 0 | 0 | 1 | 8.4 | 10
|
| 8/20 J23100-RBS-GFP-DT(334μg/ml) | EcoRI | SpeI | 10x buffer H | MilliQ | total
|
| 3.0 | 1 | 1 | 3 | 22 | 30
|
| 0.3 | 0.2 | 0 | 1 | 8.5 | 10
|
| 0.3 | 0 | 0.2 | 1 | 8.5 | 10
|
| 0.3 | 0 | 0 | 1 | 8.7 | 10
|
not written yet
Transformation
Electrophoresis
"
