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Team:DTU-Denmark/Experiments
From 2013.igem.org
Experiments
Toxicity of Intermediates
Description: This project is to determine whether the non-gaseous intermediates (nitrite, nitrate) and ammonium are toxic to E. coli, and at what concentration. This experiment will use the biolector to grow E. coli in the presence of increasing concentrations of these ions and determine their OD and fluorescence.
Verify nitrite stability in anaerobic, untransformed E. coli
Description: We grew E. coli anaerobically in the presence of varying concentrations of nitrite and measured the amount of ammonia and nitrite present.
Measure production of N2O from Nitrite NO2- anaerobically
Description: We grew E. coli and P.aeroginosa anaerobically in the presence of varying concentrations of nitrite and measured the amount of nitrous oxide produced.
