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18/07/13
From 2013.igem.org
Revision as of 14:14, 22 August 2013 by AliceHaworth (Talk | contribs)
Single digest
- Digesting RFP plasmid again using a single restriction enzyme- XbaI
- Digesting DNA samples 1-6
- 200ng of DNA is run
- Volumes for digest:
| Sample nr | 1 | 2 | 3 | 4 | 5 | 6 |
| DNA | 0.7 | 0.7 | 0.8 | 1.3 | 1.2 | 2.6 |
| Buffer | 2 | 2 | 2 | 2 | 2 | 2 |
| Water | 16.3 | 16.3 | 16.2 | 15.7 | 15.8 | 14.4 |
| XbaI | 1 | 1 | 1 | 1 | 1 | 1 |
- Buffer used was cutsmart
- Incubate samples at 37C for 1h
- Heat kill for 5min at
