Notebook : July 4

Summary:

• calculated the size of segments for bands of electrophoresis by using Clone Manager.
• Made interpretation for electrophoresis. The picture shows that the experimental result was coherent with our estimation. We ensure that we had our first BrioBrick, BBa_K1155000 (fnr+plasmid PSB1C3).
• stored 2 colonies who contain BioBrick BBa_K1155000
• As the Bi.oBrick BBa_k1155000 was confirmed, the plasmid DNA extraction was performed
• Received the bacterial strain: pseudomonas KE707.
• The extract of plasmid DNA which contain BBa_K1155000 was digested by Not I, Mlu I, Hpa I, and were amplified by PCR.
• Seeded the 4 additional broths (2 for amilCP, 2 for FNR) for plasmid extraction).

Lab work

constructing