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From 2013.igem.org

2013-08-12

• sequences arrived: File:PIK1.2-pIK2.2 seq 2013-08-12.zip
• perform multiple sequence alignment against assumed sequences: pIK1.2 with VF2, pIK1.2 with VR, pIK2.2 with VF2, pIK2.2 with VR
• pIK1.2 looks good
• part of RBS missing in pIK2.2 -> makes no sense, as part of binding part of IK35, with which the backbone was amplified
• send pIK2.6 to sequencing with primers VF2 and VR, also sequence pIK1.2 with primer IK25 to find out whether RBS is also missing there

2013-08-13

• sequences arrived: File:PIK1.2-pIK2.6 seq 2013-08-13.zip
• perform multiple sequence alignment against assumed sequences: pIK1.2 with IK25, pIK2.6 with VF2, pIK2.6 with VR
• sequences look good
• prepare glycerol stock of TOP10-pIK1.2

2013-08-14

• prepare glycerol stock of TOP10-pIK2.6