Team:MIT/ResultsBiobricks

From 2013.igem.org

iGEM 2012

Overview

  • Project Overview

miRNA Signal

  • Overview
  • siRNA Characterization
  • Exosome Isolation and Co-Culturing
  • Cell-Cell Co-Culturing

Protein Signals

  • Overview
  • GFP
  • rtTA3
  • Cre
  • L7Ae
  • Cas9-VP16

Novel DNA Sensor: Cas9 Split Venus Fusion

  • Overview
  • Leucine Zipper Fusion
  • DNA Sensing

Our BioBricks

  • Favorites
  • All BioBricks

Attributions

  • Attributions

Favorite Parts:

CMV_Acyl-TyA-eGFP MammoBlock

  • ID: BBa_K1179071
  • Type: Generator
  • Length: 2834
  • Description/Usage: This part encodes the expression of green fluorescent protein that is exported out of the cell from the addition of the acylation tag and TyA sequence that is fused to the GFP coding sequence.

hEF1a_Cas9-VP16 MammoBlock

  • ID: BBa_K1179002
  • Type: Generator
  • Length: 5141
  • Description/Usage: This part encodes the expression of the fusion protein Cas9-VP16 that is used in activating a minimal CMV promoter with guide RNA binding sites upstream.

hEF1a_eYFP-4x451 MammoBlock

  • ID: BBa_K1179072
  • Type: Generator
  • Length: 2085
  • Description/Usage: This part encodes the expression of yellow fluorescent protein and it also has four miRNA451 target sites to allow for translational repression.

All Parts

Acyl-TyABBa_K1179000
Acyl-TyA-eGFPBBa_K1179001
hEF1a_Cas9-VP16BBa_K1179002
Acyl-TyA-mKate-HisBBa_K1179003
Acyl-TyA-rtTABBa_K1179004
Acyl-TyA-rtTA-HisBBa_K1179005
Acyl-TyA-TevBBa_K1179006
Acyl-TyA-TEV-rtTA-HisBBa_K1179007
Cas9 D10A & H840A mutation-linkerBBa_K1179008
Cas9-Linker-VP16BBa_K1179009
eBFPBBa_K1179010
eYFPBBa_K1179012
eYFP-PESTBBa_K1179013
VP16-GAL4BBa_K1179014
U6_gRNA (Cr9)BBa_K1179015
L7AeBBa_K1179016
Linker-Cas9 D10A & H840A mutationBBa_K1179017
mKate –PESTBBa_K1179018
mKate-HisBBa_K1179019
rtTA-HisBBa_K1179020
CAG_Acyl-TyA-mKate-HisBBa_K1179021
CAG_Acyl-TyA-rtTABBa_K1179022
CAG_Acyl-TyA-rtTA-HisBBa_K1179023
CAG_eYFP-PESTBBa_K1179024
CAG_L7AeBBa_K1179025
CAG_mKate-PESTBBa_K1179026
CAG-tagBFPBBa_K1179027
D10A and H840A mutant Cas9-VC155BBa_K1179028
D10A and H840A mutant Cas9-VN155BBa_K1179029
D10A and H840A mutant Cas9-VN173BBa_K1179030
CMV_ L7Ae_myc-His6BBa_K1179032
CMV_2xKMet-eGFPBBa_K1179033
L7Ae-2a-eYFPBBa_K1179037
TRE_Cas9-VP16BBa_K1179040
U6_gRNA 1A ReverseBBa_K1179041
U6_gRNA 1ABBa_K1179042
U6_gRNA 2ABBa_K1179043
U6_gRNA 3ABBa_K1179044
U6_gRNA 4ABBa_K1179045
U6_gRNA 5ABBa_K1179046
U6_gRNA 6ABBa_K1179047
U6_gRNA 6A ReverseBBa_K1179048
VC155BBa_K1179049
VC155 with Leucine zipperBBa_K1179050
VC155-Cas9(D10A and H840A mutant)BBa_K1179051
VN155BBa_K1179052
VN155 with Leucine zipperBBa_K1179053
VN155-Cas9(D10A and H840A mutant)BBa_K1179054
VN173BBa_K1179055
VN173 with Leucine zipperBBa_K1179056
VN173-Cas9(D10A and H840A mutant)BBa_K1179057
Cre entry vectorBBa_K1179058
eYFP-4x451tsBBa_K1179060
eYFP-4x503tsBBa_K1179061
LacIBBa_K1179062
LacI-4x451tsBBa_K1179063
LacI-4x503tsBBa_K1179064
TagBFPBBa_K1179068
CAGBBa_K1179069
CMV_Acyl-TyA-eGFPBBa_K1179071
hEF1a_eYFP-4x451BBa_K1179072
TRE-tight_eYFP-4x451BBa_K1179073
hEF1a_eYFP-4x503BBa_K1179074
TRE-t_eYFP-4x503BBa_K1179075

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