"

From 2013.igem.org

Mirjam, Chaline and Inne
A couple of media are prepared:
LB medium with agar 1 liter
LB medium divided into 80 ml portions
70% ethanol
2 liter TBE buffer

Both LB media are autoclaved together with tooth picks, 1.5 and 2 ml eppendorf tubes. After autoclaving the LB medium with agar is placed in the 55 degrees Celsius stove.

Mirjam and Claudio
B. subtilis strain 168 is inoculated O/N at 37 degrees Celsius and every buffer necessary for the extraction of genomic DNA is arranged.