Team:Groningen/Labwork/9 July 2013

Mirjam

PCR made for silk without strep-tags.
gel run for 22 min 90V.
Gel reveals a small band at 900bp. That is why a new 1.5% agarosegel is run for 30 min at 90V.
Cut wrong bands from gel. So a new PCR is made.

Inne

spotted transformed cells and put them in a 37 C shaker (at 11:35) Cells need to grow over night. 15:00 Did a PCR in order to get more silk gene to cut out of gel(last one was cut to low)