Team:Heidelberg/Templates/Delftibactin week1
From 2013.igem.org
Contents |
29-04 - 05-05-13
Reproduction of Johnsson et al. Paper
Aim
Reproduction of Johnsson et al. paper: Precipitating gold with D.acidovorans on agar plate, in culture, the medium of the culture and possibly purified delftibactin.
Preparations
- Obtain D. acidovorans from DSMZ
- Prapare ACM medium as described
- Prepare ACM agar plates
Gold Precipitation on ACM Plates
- Culture D. acidovorans for 3 days on agar plates at 30°C
- Overlay agar plates with 0.2% HAuCl4 in 0.5 % soft agarose
Result
- Gold precipitation on ACM agar plate worked even better than described in paper
Gold Precipitation in D. acidovorans Supernatants
- Culture D. acidovorans for 3 days in ACM plates
- Centrifuge bacteria in liquid culture (10 min 6,000 rpm)
- Add 0.2% HAuCl4 to supernatant and medium control
Result
- Gold precipitation with D. acidovorans supernatant worked well
Gold Precipitation in D. acidovorans Supernatants at different Gold Chloride Concentrations
- Culture D. acidovorans for 3 days in ACM plates
- Centrifuge bacteria in liquid culture (10 min 6,000 rpm)
- Add HAuCl4 to supernatant and medium control
Result
- Gold precipitation with supernatant worked at very low concentration Gold
- Gold precipitation with unpurified culture worked as well